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The most important aspect of using pin
tools is to start with clean pins and then keep them
clean so they will consistently deliver the same volume. We
recommend our Pin Cleaning Solution (VP
110) to clean the pin of protein and other residue buildup before
you start an assay. To quickly and efficiently remove the
previous transfer from the pin we have several different wash
reservoirs, flowing reservoirs
and fountains to clean 96, 384 and 1536 floating pin tools.
These wash stations also are adapted so the
blotting station
is part of the
washing station thus reducing the cleaning cycle time and the number of
robot
stations required for the cleaning process. We also have other
independent
blotting stations (VP 540D)
Integral to the cleaning process of all
pins is the blotting
step between baths. Lint particles in the slots or on pin tips,
from standard paper towels, will interfere with the loading and
unloading of
liquid. We have solved this problem. The
VP 540DB
blotting system is a tray with the standard microplate foot print that
contains a super absorbent polypropylene pad covered by a
Lint Free Blotting Paper. We strongly recommend that
you use this or our other
lint free
blotting systems.
For chemical resistance properties of
Polypropylene and other commonly used plastics follow
this link.
We have developed
detailed procedures for cleaning pin tools
on robot systems.
Data demonstrating
thoroughness of wash procedure on carry over of
compound after 100 transfers with a slot pin. |