Product Reports

The following are reports pertaining to V&P Scientific products.

 
Manifolds
 

• Products: VP 177AD-1 , VP 179BJD
  
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  An Economical Alternative for Rapidly Filling Microplates with V&P Scientific 96 and 384 Dispensing Manifolds
   

  White Paper by Duong Chau V&P Scientific Staff Scientist
   

  Abstract
  V&P Scientific VP 177AD-1 and VP 179BJD are dispensing manifolds designed for rapid filling of 96 and 394 well microplates respectively.  This experiment was set up to determine both the accuracy and precision of these instruments by measuring the quantity of aqueous FITC dispensed per well and calculating the coefficient of variation among the wells and also the variation among the dispenses.
  
  The manifolds were tested in volumes of 50, 100, and 200 uL per well for the VP 177AD-1 and 25, 50, and 75 uL per well for the VP 179BJD.  The coefficient of variations ranged between 3% and 7% using the VP 177AD-1 and between 4% and 5% using the VP 179BJD when dispensing occurred below the meniscus in contact with the liquid.  Non-contact dispensing was examined using the VP 179BJD and resulted in coefficient of variations ranging between 6% and 9%.
  
  The VP 177AD-1 and VP 179BJD manifolds are capable of rapid dispensing into microplates.  These instruments can instantly fill a microplate with one dispense, while maintaining acceptable precision.  Standard pipettors often require multiple dispenses to fill a microplate, twelve dispenses for a 96 well microplate and thirty-two dispenses for a 384 well microplate using a 12-channel pipettor.  This makes the VP 177AD-1 and VP 179BJD a convenient alternative to regular pipetting, or an economical replacement for expensive workstations.

• Products: VP 180, VP 184, VP 189L, VP 189M
  
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  Precision of Dispensing Using V&P Wands and Manifolds

  White Paper by Duong Chau V&P Scientific Staff Scientist
   

  Abstract
  V&P Scientific wands and manifolds are designed for the purpose of aspirating from microplates, washing microplates, and dispensing into microplates.  This experiment was set up to determine the precision of the latter capabilities by measuring the quantity of aqueous FITC dispensed per well and calculating the coefficient of variations among the wells as well as among the dispenses.

  The wands and manifolds range between 4 and 48 tubes and are designed for microplates with wells between 48 and 1536.  This assay evaluated the performance of four separate devices, the VP 180, VP 184 (96 well format), VP 189L, and VP 189M (384 well format); devices that represent the fundamental design of the wands and manifolds.  The experiment also provided the data necessary to determine if the design differences between wands and manifolds affect the dispense performance.

  Dispensing was done in a non-contact fashion (above the wells) and generally resulted in coefficient of variations below 5% for all devices tested.  It was also observed that despite the differences between the wands and manifolds in design, there were no apparent differences in the quality of dispensing capabilities.  Only four devices out of many different possible wand and manifold products V&P Scientific carries were tested.  However, this assay provides a proof of principle for the design of the wands and manifolds, thus similarly designed products are expected to yield similar results.